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Valiant Co Ltd rapid immunoprecipitation assay buffer
Rapid Immunoprecipitation Assay Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+immunoprecipitation+assay+buffer/Tris+hydrochloride%2C+ultra+pure/pm41701515-513-17-27
Average 94 stars, based on 22 article reviews
rapid immunoprecipitation assay buffer - by Bioz Stars, 2026-11
94/100 stars

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Isolation:

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulatepollen tube growth.
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μL of rapid immunoprecipitation assay buffer (RIPA: 10 mM Tris/HCl pH 7.5 [MP Biomedicals], 150 mM NaCl [Sigma], 0.5 mM EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 mM phenylmethylsulphonyl fluoride [Sigma]). ..

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis -elements to regulate pollen tube growth
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μ L of rapid immunoprecipitation assay buffer (RIPA: 10 m m Tris/HCl pH 7.5 [MP Biomedicals], 150 m m NaCl [Sigma], 0.5 m m EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 m m phenylmethylsulphonyl fluoride [Sigma]). ..

Immunoprecipitation:

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulatepollen tube growth.
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μL of rapid immunoprecipitation assay buffer (RIPA: 10 mM Tris/HCl pH 7.5 [MP Biomedicals], 150 mM NaCl [Sigma], 0.5 mM EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 mM phenylmethylsulphonyl fluoride [Sigma]). ..

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis -elements to regulate pollen tube growth
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μ L of rapid immunoprecipitation assay buffer (RIPA: 10 m m Tris/HCl pH 7.5 [MP Biomedicals], 150 m m NaCl [Sigma], 0.5 m m EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 m m phenylmethylsulphonyl fluoride [Sigma]). ..

Protease Inhibitor:

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulatepollen tube growth.
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μL of rapid immunoprecipitation assay buffer (RIPA: 10 mM Tris/HCl pH 7.5 [MP Biomedicals], 150 mM NaCl [Sigma], 0.5 mM EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 mM phenylmethylsulphonyl fluoride [Sigma]). ..

Article Title: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis -elements to regulate pollen tube growth
Article Snippet: .. Total protein from soluble fractions was isolated by grounding pellet in liquid nitrogen with 250 μ L of rapid immunoprecipitation assay buffer (RIPA: 10 m m Tris/HCl pH 7.5 [MP Biomedicals], 150 m m NaCl [Sigma], 0.5 m m EDTA [Duchefa], 0.5% NP-40 [Sigma], pierce protease inhibitor [Thermo Fisher Scientific, 1 tablet per 30 mL buffer], 10 m m phenylmethylsulphonyl fluoride [Sigma]). ..



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